The Expression of Connexin 26 Regulates the Radiosensitivity of Hepatocellular Carcinoma Cells through a Mitogen-Activated Protein Kinases Signal Pathway.
Yuan LiLi YangRui TaoYajing ShangMinqiong SunShichao PengGuoping ZhaoYe ZhaoPublished in: International journal of molecular sciences (2022)
Connexin 26 (Cx26) is a protein that constitutes a gap junction and is widely expressed in the liver. Abnormal expression of Cx26 is one of the important mechanisms of liver cancer, and is closely related to the transmission of radiation damage signals between cells. In the present study, we investigated the radiosensitivity of hepatocellular carcinoma (HCC) cells HepG2, with low expression of Cx26, and SK-hep-1, with high expression of Cx26 after X-ray irradiation. The cell survival, micronucleus formation and protein expressions of the mitogen-activated protein kinases (MAPK) signaling pathway were detected. The expression level of Cx26 could affect the radiosensitivity of liver cancer cells by affecting the phosphorylation of p38 and ERK proteins and regulating the expression of downstream NF-κB. Cell lines with knock-out and overexpression of Cx26 were also built to confirm the findings. Our results suggested that Cx26 might play an important role in the radiosensitivity of liver cancer and could be a potential target for clinical radiotherapy of liver cancer.
Keyphrases
- poor prognosis
- signaling pathway
- binding protein
- induced apoptosis
- pi k akt
- oxidative stress
- cell cycle arrest
- cell proliferation
- immune response
- magnetic resonance imaging
- mass spectrometry
- risk assessment
- cell death
- transcription factor
- human health
- small molecule
- rectal cancer
- inflammatory response
- locally advanced
- protein protein
- lps induced