Ascophyllan Induces Activation of Natural Killer Cells in Mice In Vivo and In Vitro.
Wei ZhangTakasi OkimuraTatsuya OdaJun-O JinPublished in: Marine drugs (2019)
Natural marine polysaccharides have demonstrated immune stimulatory effects in both mice and humans. Our previous study compared the ability of ascophyllan and fucoidan to activate human and mouse dendritic cells (DCs). In this study, we further examined the effect of ascophyllan on the activation of mouse natural killer (NK) cells in vivo and in vitro and compared it to that of fucoidan, a well-studied natural marine polysaccharide. Specifically, administration of ascophyllan to C57BL/6 mice increased the number of NK cells in the spleen when compared to the number in PBS-treated mice. Moreover, the number of IFN-γ-producing NK cells and expression of CD69 were markedly upregulated by ascophyllan treatment. Ascophyllan treatment also induced IFN-γ production and CD69 upregulation in isolated NK cells, but did not promote cell proliferation. Finally, ascophyllan treatment increased the cytotoxicity of NK cells against Yac-1 cells. The effects of ascophyllan on NK cell activation were considerably stronger than those of fucoidan. These data demonstrated that ascophyllan promotes NK cell activation both in mice and in vitro, and its stimulatory effect on NK cells is stronger than that of fucoidan.
Keyphrases
- nk cells
- dendritic cells
- high fat diet induced
- cell proliferation
- immune response
- poor prognosis
- endothelial cells
- natural killer cells
- induced apoptosis
- oxidative stress
- type diabetes
- combination therapy
- insulin resistance
- cell death
- skeletal muscle
- metabolic syndrome
- machine learning
- diabetic rats
- cell cycle arrest
- long non coding rna
- endoplasmic reticulum stress
- big data