Enhancing Bioactive Components of Euryale ferox with Lactobacillus curvatus to Reduce H 2 O 2 -Induced Oxidative Stress in Human Skin Fibroblasts.
Yanbing JiangShiquan YouYongtao ZhangJingsha ZhaoDongdong WangDan ZhaoMeng LiChangtao WangPublished in: Antioxidants (Basel, Switzerland) (2022)
This study investigated the effects of Lactobacillus curvatus fermentation on the oxidative stress attenuating effects of Euryale ferox on H 2 O 2 -induced human skin fibroblasts (HSF). The results showed that Lactobacillus curvatus fermentation (i) increases the content of the various bioactive components of Euryale ferox and is found to have smaller molecular weights of polysaccharides and polypeptides; (ii) increases the overall intracellular and extracellular antioxidant capacity of H 2 O 2 -induced HSF while reducing reactive oxygen species (ROS) levels. Superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) all showed simultaneous increases in activity. Aside from that, the Nrf2 and MAPK signaling pathways are activated to regulate downstream-associated proteins such as the Bax/Bcl-2 protein ratio, matrix metalloproteinase 1 (MMP-1) activity, and human type I collagen (COL-1). These results suggested that the fermentation of Euryale ferox with Lactobacillus curvatus enhances its antioxidant capacity and attenuates apoptosis and senescence caused by oxidative stress.
Keyphrases
- oxidative stress
- diabetic rats
- lactic acid
- reactive oxygen species
- dna damage
- induced apoptosis
- endothelial cells
- heat shock
- high glucose
- hydrogen peroxide
- signaling pathway
- ischemia reperfusion injury
- saccharomyces cerevisiae
- cell death
- endoplasmic reticulum stress
- drug induced
- extracellular matrix
- high resolution
- epithelial mesenchymal transition
- tissue engineering
- protein protein