Use of the rhizobial type III effector gene nopP to improve Agrobacterium rhizogenes-mediated transformation of Lotus japonicus.

Yan WangFeng YangPeng-Fei ZhuAsaf KhanZhi-Ping XieChristian Staehelin

Published in: Plant methods (2021)

We have established a novel protocol for hairy root transformation of L. japonicus. The use of A. rhizogenes LBA9402 carrying a binary vector containing DsRed1 and nopP allowed efficient formation and identification of transgenic roots.

Keyphrases

type iii
randomized controlled trial
genome wide
copy number
ionic liquid
dendritic cells
immune response
transcription factor
regulatory t cells