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HMGN1 enhances CRISPR-directed dual-function A-to-G and C-to-G base editing.

Chao YangZhenzhen MaKeshan WangXingxiao DongMeiyu HuangYaqiu LiXiagu ZhuJu LiZhihui ChengZaiqiang WuXueli Zhang
Published in: Nature communications (2023)
C-to-G base editors have been successfully constructed recently, but limited work has been done on concurrent C-to-G and A-to-G base editing. In addition, there is also limited data on how chromatin-associated factors affect the base editing. Here, we test a series of chromatin-associated factors, and chromosomal protein HMGN1 was found to enhance the efficiency of both C-to-G and A-to-G base editing. By fusing HMGN1, GBE and ABE to Cas9, we develop a CRISPR-based dual-function A-to-G and C-to-G base editor (GGBE) which is capable of converting simultaneous A and C to G conversion with substantial editing efficiency. Accordingly, the HMGN1 role shown in this work and the resulting GGBE tool further broaden the genome manipulation capacity of CRISPR-directed base editors.
Keyphrases
  • crispr cas
  • genome editing
  • genome wide
  • gene expression
  • dna damage
  • transcription factor
  • squamous cell carcinoma
  • dna methylation
  • machine learning
  • amino acid