Increased Glucosinolate Production in Brassica oleracea var. italica Cell Cultures Due to Coronatine Activated Genes Involved in Glucosinolate Biosynthesis.
P J Sánchez-PujanteA B Sabater-JaraS Belchí-NavarroM A PedreñoLorena AlmagroPublished in: Journal of agricultural and food chemistry (2018)
In this work, the effect of different elicitors and culture conditions on the production of glucosinolates in broccoli cell cultures was studied. The results showed that 0.5 μM coronatine was the best elicitor for increasing glucosinolate production (205-fold increase over untreated cells after 72 h of treatment). Furthermore, the expression levels of some genes related to the biosynthetic pathway of glucosinolates as well as three Myb transcription factors also have been studied. The highest glucosinolate levels found in coronatine-treated cells were closely correlated with the highest gene expression levels of Cyp79b2, Cyp83b1, St5a, Myb51, and Myb122 after 6 h of treatment. The data shown in this study provide new insight into the key metabolic steps involved in the biosynthesis of glucosinolates, which will be of use for future applications of metabolic engineering techniques in broccoli.
Keyphrases
- transcription factor
- gene expression
- induced apoptosis
- cell cycle arrest
- single cell
- cell therapy
- genome wide identification
- poor prognosis
- dna methylation
- stem cells
- endoplasmic reticulum stress
- combination therapy
- big data
- current status
- machine learning
- dna binding
- pi k akt
- long non coding rna
- smoking cessation
- solid state