Pseudorabies virus (PRV) causes high mortality and miscarriage rates in the infected swine, and the eradication policy coupled with large-scale vaccination of live attenuated vaccines has been adopted globally against PRV. Differential diagnosis of the vaccinated and infected swine is highly demanded. Our multienzyme isothermal rapid amplification (MIRA)-Cas12a detection method described in this study can diagnose PRV with a superior sensitivity comparable to the quantitative PCR (qPCR) and a competitive detection speed (only half the time as qPCR needs). The portable feature and the simple procedure of MIRA-Cas12a make it easier to deploy for clinical diagnosis, even in resource-limited settings. The MIRA-Cas12a method would provide immediate and accurate diagnostic information for policymakers to respond promptly.
Keyphrases
- crispr cas
- genome editing
- loop mediated isothermal amplification
- real time pcr
- label free
- public health
- healthcare
- high resolution
- machine learning
- nucleic acid
- mental health
- deep learning
- cardiovascular events
- cardiovascular disease
- sensitive detection
- minimally invasive
- health information
- social media
- disease virus