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Establishment of monoclonal antibodies broadly neutralize infection of hepatitis B virus.

He ZhangYumi ItohTatsuya SuzukiKan-Ichiro IharaTomohisa TanakaSaori HagaHajime EnatsuMaho YumiyaMari KimuraAkira TakadaDaiki ItohYuri ShibazakiShuto NakaoSachiyo YoshioKei MiyakawaYuki MiyamotoHanae SasakiTadahiro KajitaMasaya SugiyamaMasashi MizokamiTaro TachibanaAkihide RyoKohji MoriishiEiji MiyoshiTatsuya KantoToru OkamotoYoshiharu Matsuura
Published in: Microbiology and immunology (2022)
Antibodies against hepatitis B virus S protein can protect against hepatitis B virus (HBV) infection. Therefore, hepatitis B immunoglobulin (HBIG), which contains HBsAb, is used clinically as a therapy for HBV infection. In this study, a series of monoclonal antibodies that recognize multiple HBV genotypes was obtained. All the antibodies recognized conformational epitopes of S protein, but not linear epitopes. Several antibodies neutralized HBV infection and exhibited strong affinities and neutralizing activities. Antigenic epitope analysis demonstrated that they recognized residue Ile152 of S protein, which is localized outside the "a" determinant. Ile152 is highly conserved, and a mutation in this residue resulted in reduced expression of large hepatitis B surface proteins (L protein), suggesting that the amino acid at this position is involved in the expression of L protein. In addition, the antibodies neutralized the infection of hepatitis D virus possessing a Gly145 mutation to Arg in S protein, which is a well-known escape mutation against HBIG treatment. Using mouse monoclonal antibodies, a humanized antibody possessing affinities and neutralizing activities similar to those of the original mouse antibody was successfully established. The antibodies generated in this study may have the potential for use in alternative antibody therapies for HBV infection.
Keyphrases
  • hepatitis b virus
  • amino acid
  • liver failure
  • protein protein
  • binding protein
  • poor prognosis
  • transcription factor
  • molecular dynamics simulations
  • long non coding rna
  • replacement therapy
  • combination therapy