OMIP-103: A 35-marker imaging mass cytometry panel for the co-detection of HIV and immune cell populations in human formalin fixed paraffin embedded intestinal tissue.
Kevin HuThomas O'NeilNicolas CaneteHeeva BaharlouAndrew N HarmanPublished in: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2024)
We introduce a 35-marker imaging mass cytometry (IMC) panel for a detailed examination of immune cell populations and HIV RNA in formalin fixed paraffin embedded (FFPE) human intestinal tissue. The panel has broad cell type coverage and particularly excels in delineating subsets of mononuclear phagocytes and T cells. Markers for key tissue structures are included, enabling identification of epithelium, blood vessels, lymphatics, and musculature. The described method for HIV RNA detection can be generalized to other low abundance RNA targets, whether endogenous or pathogen derived. As such, the panel presented here is useful for high parameter spatial mapping of intestinal immune cells and their interactions with pathogens such as HIV.
Keyphrases
- antiretroviral therapy
- hiv positive
- hiv testing
- hiv infected
- human immunodeficiency virus
- high resolution
- hepatitis c virus
- hiv aids
- men who have sex with men
- endothelial cells
- south africa
- single cell
- healthcare
- pluripotent stem cells
- induced pluripotent stem cells
- peripheral blood
- mass spectrometry
- nucleic acid
- wastewater treatment
- high density