Discovery of prescopranone, a key intermediate in scopranone biosynthesis.
Ayumu DemachiSatoshi OhteRyuji UchidaTeppei KawaharaTaichi OhshiroHiroshi TomodaHaruo IkedaPublished in: The Journal of antibiotics (2022)
A key intermediate in scopranone biosynthesis, prescopranone, accumulated in the mycelium of Streptomyces avermitilis SUKA carrying the biosynthetic gene cluster for scopranone lacking the sprT encoding the monooxygenase. The structure of prescopranone was elucidated by NMR and other spectral data. Prescopranone consists of a 2-pyranone ring with two atypical scoop-like moieties (1-ethyl-1-propenyl and 2-ethylbutyl groups), which was deduced as a product of the modular polyketide syntheses encoded by sprA, sprB, and sprC. Prescopranone inhibited bone morphogenetic protein (BMP)-induced alkaline phosphatase activity in a BMP receptor mutant cell line.
Keyphrases
- mesenchymal stem cells
- cell wall
- high glucose
- bone regeneration
- magnetic resonance
- small molecule
- diabetic rats
- optical coherence tomography
- high resolution
- electronic health record
- genome wide
- high throughput
- big data
- drug induced
- gene expression
- solid state
- computed tomography
- oxidative stress
- dna methylation
- binding protein
- bone marrow
- single cell
- genome wide identification
- dual energy