Optimization of a decellularization protocol of porcine tracheas. Long-term effects of cryopreservation. A histological study.

The results presented here reinforce the use of SDS as a valuable decellularizing agent for porcine tracheas. Furthermore, a cryogenic preservation protocol is described, which has minimal impact on the histological and biomechanical properties of decellularized porcine tracheas.