cGMP-dependent kinase 2, Na + /H + exchanger NHE3, and PDZ-adaptor NHERF2 co-assemble in apical membrane microdomains.

NHE3, cGKII, and NHERF2 form a lipid raft-associated signal complex in the siBBM, which mediates the inhibition of salt and water absorption by Gucy2c activation. NHERF2 enhances the raft association of NHE3, which is essential for its close interaction with the exclusively raft-associated activated cGKII.