Validation of proteins associated with pathological damage in human tuberculosis granulomas: study protocol.
Thabo MpotjeJessica MoreKerishka Rajkumar-BhugelooDenelle MoodleyMohlopheni J MarakalalaPublished in: Wellcome open research (2023)
The presence of the Tuberculosis (TB) disease-causing pathogen, Mycobacterium Tuberculosis (Mtb), induces the development of a pathological feature termed granuloma, which the host uses to contain the bacteria. However, the granuloma may dissociate resulting in detrimental caseation of the lung. The disease contributes to a growing global burden of lung function challenges, warranting for more understanding of the TB-induced immunopathology. The current study aims to explore in detail host factors that drive pathological features of TB contributing to extensive lung tissue destruction. Lung tissue sections obtained from patients undergoing surgical resection will be processed and analyzed using histopathological assays including Immunohistochemistry, Immunofluorescence, Hematoxylin and Eosin staining and Laser Capture Microdissection. The findings will provide key host factors that associate with exacerbated lung immunopathology during TB.
Keyphrases
- mycobacterium tuberculosis
- pulmonary tuberculosis
- lung function
- patients undergoing
- study protocol
- randomized controlled trial
- endothelial cells
- cystic fibrosis
- chronic obstructive pulmonary disease
- air pollution
- clinical trial
- high throughput
- high glucose
- candida albicans
- machine learning
- open label
- oxidative stress
- risk factors