Single-cell sequencing of human white adipose tissue identifies new cell states in health and obesity.
Andrew D HildrethFeiyang MaYung Yu WongRyan SunMatteo PellegriniTimothy E O'SullivanPublished in: Nature immunology (2021)
White adipose tissue (WAT) is an essential regulator of energy storage and systemic metabolic homeostasis. Regulatory networks consisting of immune and structural cells are necessary to maintain WAT metabolism, which can become impaired during obesity in mammals. Using single-cell transcriptomics and flow cytometry, we unveil a large-scale comprehensive cellular census of the stromal vascular fraction of healthy lean and obese human WAT. We report new subsets and developmental trajectories of adipose-resident innate lymphoid cells, dendritic cells and monocyte-derived macrophage populations that accumulate in obese WAT. Analysis of cell-cell ligand-receptor interactions and obesity-enriched signaling pathways revealed a switch from immunoregulatory mechanisms in lean WAT to inflammatory networks in obese WAT. These results provide a detailed and unbiased cellular landscape of homeostatic and inflammatory circuits in healthy human WAT.
Keyphrases
- single cell
- adipose tissue
- insulin resistance
- rna seq
- weight loss
- metabolic syndrome
- endothelial cells
- type diabetes
- dendritic cells
- induced apoptosis
- high throughput
- high fat diet
- high fat diet induced
- flow cytometry
- bariatric surgery
- public health
- oxidative stress
- cell cycle arrest
- healthcare
- induced pluripotent stem cells
- bone mineral density
- weight gain
- cell therapy
- pluripotent stem cells
- social media
- bone marrow
- mental health
- immune response
- quality improvement
- cell death
- body composition
- health information
- risk assessment
- genetic diversity
- epithelial mesenchymal transition