Evaluation of hematology, general serum biochemistry, bone turnover markers and bone marrow cytology in a glucocorticoid treated ovariectomized sheep model for osteoporosis research.
Catarina A CoelhoJoÃo P BordeloJosé Arthur CamassaVera A BarrosPedro S BaboManuela Estima GomesRui Luis ReisJorge T DE AzevedoJoão Filipe RequichaPedro FaíscaPedro Pires CarvalhoCarlos A ViegasIsabel R DiasPublished in: Anais da Academia Brasileira de Ciencias (2020)
Osteoporosis is a metabolic disorder characterized by a loss of bone mass and structure and increasing the risk of fragility fractures, mostly among postmenopausal women. Sheep is a recognized large animal model for osteoporosis research. An experimental group of ewes (3-4 years old) was subjected to ovariectomy (OVX) and weekly glucocorticoid (GC) application for 24 weeks and compared with a sham control group. Blood and bone marrow parameters were analyzed before and 24 weeks after OVX and GC administration. Osteopenia was confirmed through micro-computed tomography and histomorphometric analysis of L4 vertebra in the study end. A statistically significant increase was observed in mean corpuscular volume, mean cell hemoglobin and monocytes and a decrease in red blood count and eosinophils (p<0.05). Alkaline phosphatase (ALP), gamma-glutamyl transpeptidase, magnesium and α1-globulin increased, and creatinine, albumin, sodium and estradiol decreased (p<0.05). A slight decrease of bone formation markers (bone ALP and osteocalcin) and an increase of bone resorption markers (C-terminal telopeptides of collagen type 1 and tartrate-resistant acid phosphatase) were observed, but without statistical significance. This study aims to contribute to better knowledge of sheep as a model for osteoporosis research and the consequences that a performed induction protocol may impose on organic metabolism.
Keyphrases
- bone mineral density
- postmenopausal women
- bone marrow
- body composition
- computed tomography
- bone loss
- mesenchymal stem cells
- healthcare
- randomized controlled trial
- magnetic resonance imaging
- soft tissue
- peripheral blood
- positron emission tomography
- immune response
- stem cells
- dendritic cells
- gestational age
- high resolution
- cell therapy
- red blood cell
- water soluble