A novel approach to carotenoid accumulation in rice callus by mimicking the cauliflower Orange mutation via genome editing.
Akira EndoHiroaki SaikaMiho TakemuraNorihiko MisawaSeiichi TokiPublished in: Rice (New York, N.Y.) (2019)
We demonstrate that directed gene modification of the Osor gene via CRISPR/Cas9-mediated genome editing results in β-carotene fortification in rice calli. To date, golden rice, which accumulates β-carotene in rice endosperm, has been developed by conventional transgenic approaches. Our results suggest an alternative approach to enhancing β-carotene accumulation in crops.