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A protocol for good quality genomic DNA isolation from formalin-fixed paraffin-embedded tissues without using commercial kits.

Fazlur Rahman TalukdarIrena AbramovićCyrille CueninChristine CarreiraNitin GanganeNino SincicZdenko Herceg
Published in: Molecular biology reports (2022)
Overall, IARCp represents a novel approach to DNA isolation from FFPE which results in good quality and significant amounts of DNA suitable for many downstream genome-wide and targeted molecular analyses. This protocol does not require the use of any commercial kits or phenol for isolating DNA from FFPE tissues, making it suitable to implement in low-resource settings such as low and middle-income countries.
Keyphrases
  • circulating tumor
  • single molecule
  • cell free
  • genome wide
  • randomized controlled trial
  • gene expression
  • nucleic acid
  • dna methylation
  • quality improvement
  • circulating tumor cells
  • copy number