Antimicrobial effects of terpinen-4-ol against oral pathogens and its capacity for the modulation of gene expression.
Ester Alves Ferreira BordiniCaroline Coradi TononRenata Serignoli FrancisconiFernando Augusto Cintra MagalhãesPatrícia Milagros Maquera HuachoTelma Blanca Lombardo BedranVanderlei S BagnatoLuis Carlos SpolidorioDenise Madalena Palomari SpolidorioPublished in: Biofouling (2018)
This study evaluated the antibacterial activity of terpinen-4-ol against Streptococcus mutans and Lactobacillus acidophilus and its influence on gbpA (S. mutans) and slpA (L. acidophilus) gene expression. As measured by XTT assay, the concentrations of terpinen-4-ol that effectively inhibited the biofilm were 0.24% and 0.95% for S. mutans and L. acidophilus, respectively. Confocal microscopy revealed the presence of a biofilm attached to the enamel and dentin block surfaces with significant terpinen-4-ol effects against these microorganisms. The expression of the gbpA and slpA genes involved in adherence and biofilm formation was investigated using RT-PCR. Expression of these genes decreased after 15 min with 0.24% and 0.95% terpinen-4-ol in S. mutans and L. acidophilus, respectively. These findings demonstrate the antimicrobial activity of terpinen-4-ol and its ability to modulate the expression of gbpA and slpA genes, emphasizing the therapeutic capacity of terpinen-4-ol as an alternative to inhibit adherence in biofilm.
Keyphrases
- biofilm formation
- candida albicans
- staphylococcus aureus
- pseudomonas aeruginosa
- gene expression
- poor prognosis
- escherichia coli
- dna methylation
- cystic fibrosis
- genome wide
- type diabetes
- metabolic syndrome
- mass spectrometry
- multidrug resistant
- single cell
- gram negative
- bioinformatics analysis
- weight loss
- glycemic control
- genome wide analysis
- high speed