Proteomic profiling of Klebsiella pneumoniae carbapenemase (KPC)-producer Klebsiella pneumoniae after induced polymyxin resistance.
Paula Assis QueirozJean Eduardo MeneguelloBruna R SilvaKatiany Rizzieri Caleffi FerracioliRegiane Bertin de Lima ScodroRosilene Fressatti CardosoRogério MarchiosiVera Lúcia Dias SiqueiraPublished in: Future microbiology (2021)
Aim: To elucidate the changes in protein expression associated with polymyxin resistance in Klebsiella pneumoniae, we profiled a comparative proteomic analysis of polymyxin B-resistant mutants KPC-2-producing K. pneumoniae, and of its susceptible counterparts. Material & methods: Two-dimensional reversed phase nano ultra-performance liquid chromatography mass spectrometry was used for proteomic analysis. Results: Our results showed that the proteomic profile involved several biological processes, and we highlight the downregulation of outer membrane protein A (OmpA) and the upregulation of SlyB outer membrane lipoprotein (conserved protein member of the PhoPQ regulon) and AcrA multidrug efflux pump in polymyxin B-resistant strains. Conclusion: Our results highlight the possible participation of the SlyB, AcrA and OmpA proteins in the determination of polymyxin B heteroresistance in KPC-2-producing K. pneumoniae.
Keyphrases
- klebsiella pneumoniae
- multidrug resistant
- gram negative
- escherichia coli
- mass spectrometry
- liquid chromatography
- drug resistant
- acinetobacter baumannii
- high resolution
- cell proliferation
- signaling pathway
- label free
- high resolution mass spectrometry
- physical activity
- tandem mass spectrometry
- poor prognosis
- transcription factor
- high glucose
- pseudomonas aeruginosa
- single cell
- endothelial cells
- capillary electrophoresis
- oxidative stress
- amino acid
- gas chromatography