Identification and characterization of a novel multi-stress responsive gene in Arabidopsis.
Faiza TawabIqbal MunirZeeshan NasimMohammad Sayyar KhanSaleha TawabAdnan NasimAqib IqbalMian Afaq AhmadWaqar AliRaheel MunirMaria MunirNoreen AsimPublished in: PloS one (2020)
Abiotic stresses especially salinity, drought and high temperature result in considerable reduction of crop productivity. In this study, we identified AT4G18280 annotated as a glycine-rich cell wall protein-like (hereafter refer to as GRPL1) protein as a potential multistress-responsive gene. Analysis of public transcriptome data and GUS assay of pGRPL1::GUS showed a strong induction of GRPL1 under drought, salinity and heat stresses. Transgenic plants overexpressing GRPL1-3HA showed significantly higher germination, root elongation and survival rate under salt stress. Moreover, the 35S::GRPL1-3HA transgenic lines also showed higher survival rates under drought and heat stresses. GRPL1 showed similar expression patterns with Abscisic acid (ABA)-pathway genes under different growth and stress conditions, suggesting a possibility that GRPL1 might act in the ABA pathway that is further supported by the inability of ABA-deficient mutant (aba2-1) to induce GRPL1 under drought stress. Taken together, our data presents GRPL1 as a potential multi-stress responsive gene working downstream of ABA.
Keyphrases
- arabidopsis thaliana
- transcription factor
- genome wide identification
- heat stress
- genome wide
- climate change
- cell wall
- copy number
- cancer therapy
- plant growth
- high temperature
- stress induced
- dna methylation
- microbial community
- electronic health record
- emergency department
- poor prognosis
- high throughput
- big data
- gene expression
- binding protein
- genome wide analysis
- human health
- single cell
- deep learning
- wild type