Preservation of the naïve features of mesenchymal stromal cells in vitro: Comparison of cell- and bone-derived decellularized extracellular matrix.
Ana Rita PereiraDrenka TrivanovićPhilipp StahlhutMaximilian RudertJürgen GrollMarietta HerrmannPublished in: Journal of tissue engineering (2022)
The fate and behavior of bone marrow mesenchymal stem/stromal cells (BM-MSC) is bidirectionally influenced by their microenvironment, the stem cell niche, where a magnitude of biochemical and physical cues communicate in an extremely orchestrated way. It is known that simplified 2D in vitro systems for BM-MSC culture do not represent their naïve physiological environment. Here, we developed four different 2D cell-based decellularized matrices (dECM) and a 3D decellularized human trabecular-bone scaffold (dBone) to evaluate BM-MSC behavior. The obtained cell-derived matrices provided a reliable tool for cell shape-based analyses of typical features associated with osteogenic differentiation at high-throughput level. On the other hand, exploratory proteomics analysis identified native bone-specific proteins selectively expressed in dBone but not in dECM models. Together with its architectural complexity, the physico-chemical properties of dBone triggered the upregulation of stemness associated genes and niche-related protein expression, proving in vitro conservation of the naïve features of BM-MSC.
Keyphrases
- extracellular matrix
- bone marrow
- stem cells
- single cell
- bone mineral density
- high throughput
- cell therapy
- mesenchymal stem cells
- endothelial cells
- cell proliferation
- mass spectrometry
- soft tissue
- physical activity
- epithelial mesenchymal transition
- genome wide
- tissue engineering
- signaling pathway
- gene expression
- poor prognosis
- bone regeneration
- clinical evaluation