Development and validation of a high-throughput calcium mobilization assay for the orphan receptor GPR88.

Ann M DeckerElaine A GayKelly M MathewsTaylor C RosaTiffany L LangstonRangan MaitraChunyang Jin

Published in: Journal of biomedical science (2017)

We have developed a high-throughput calcium mobilization assay for the orphan receptor GPR88. This calcium mobilization assay can be used to identify several different types of GPR88 ligands including agonists, competitive and noncompetitive antagonists, inverse agonists, and allosteric modulators. These ligands will serve as valuable tools to probe signaling mechanisms and in vivo functions of GPR88, and could expedite development of novel therapies for diseases potentially mediated by GPR88.

Keyphrases

high throughput
fatty acid
single cell
small molecule
binding protein
living cells