Regeneration of Non-Alcoholic Fatty Liver Cells Using Chimeric FGF21/HGFR: A Novel Therapeutic Approach.
Sung-Jun KimSo-Jung KimJeongeun HyunHae-Won KimJun-Hyeog JangPublished in: International journal of molecular sciences (2024)
Non-alcoholic fatty liver disease (NAFLD) has emerged as a significant liver ailment attributed to factors like obesity and diabetes. While ongoing research explores treatments for NAFLD, further investigation is imperative to address this escalating health concern. NAFLD manifests as hepatic steatosis, precipitating insulin resistance and metabolic syndrome. This study aims to validate the regenerative potential of chimeric fibroblast growth factor 21 (FGF21) and Hepatocyte Growth Factor Receptor (HGFR) in NAFLD-afflicted liver cells. AML12, a murine hepatocyte cell line, was utilized to gauge the regenerative effects of chimeric FGF21/HGFR expression. Polysaccharide accumulation was affirmed through Periodic acid-Schiff (PAS) staining, while LDL uptake was microscopically observed with labeled LDL. The expression of FGF21/HGFR and NAFLD markers was analyzed by mRNA analysis with RT-PCR, which showed a decreased expression in acetyl-CoA carboxylase 1 ( ACC1 ) and sterol regulatory element binding protein ( SREBP ) cleavage-activating protein ( SCAP ) with increased expression of hepatocellular growth factor ( HGF ), hepatocellular nuclear factor 4 alpha ( HNF4A ), and albumin ( ALB ). These findings affirm the hepato-regenerative properties of chimeric FGF21/HGFR within AML12 cells, opening novel avenues for therapeutic exploration in NAFLD.
Keyphrases
- growth factor
- cell therapy
- binding protein
- stem cells
- metabolic syndrome
- poor prognosis
- induced apoptosis
- insulin resistance
- nuclear factor
- type diabetes
- mesenchymal stem cells
- cell cycle arrest
- acute myeloid leukemia
- liver injury
- public health
- cardiovascular disease
- toll like receptor
- adipose tissue
- long non coding rna
- healthcare
- signaling pathway
- mental health
- weight loss
- small molecule
- cell proliferation
- skeletal muscle
- tissue engineering
- body mass index
- oxidative stress
- computed tomography
- polycystic ovary syndrome
- weight gain
- pet imaging
- ultrasound guided