Static Magnetic Field Reduces Intracellular ROS Levels and Protects Cells Against Peroxide-Induced Damage: Suggested Roles for Catalase.
Emilli Caroline Garcia FrachiniJean Bezerra SilvaBarbara FornaciariMauricio da Silva BaptistaHenning UlrichDenise Freitas Siqueira PetriPublished in: Neurotoxicity research (2023)
A feature in neurodegenerative disorders is the loss of neurons, caused by several factors including oxidative stress induced by reactive oxygen species (ROS). In this work, static magnetic field (SMF) was applied in vitro to evaluate its effect on the viability, proliferation, and migration of human neuroblastoma SH-SY5Y cells, and on the toxicity induced by hydrogen peroxide (H 2 O 2 ), tert-butyl hydroperoxide (tBHP), H 2 O 2 /sodium azide (NaN 3 ) and photosensitized oxidations by photodynamic therapy (PDT) photosensitizers. The SMF increased almost twofold the cell expression of the proliferation biomarker Ki-67 compared to control cells after 7 days of exposure. Exposure to SMF accelerated the wound healing of scratched cell monolayers and significantly reduced the H 2 O 2 -induced and the tBHP-induced cell deaths. Interestingly, SMF was able to revert the effects of NaN 3 (a catalase inhibitor), suggesting an increased activity of catalase under the influence of the magnetic field. In agreement with this hypothesis, SMF significantly reduced the oxidation of DCF-H2, indicating a lower level of intracellular ROS. When the redox imbalance was triggered through photosensitized oxidation, no protection was observed. This observation aligns with the proposed role of catalase in cellular proctetion under SMF. Exposition to SMF should be further validated in vitro and in vivo as a potential therapeutic approach for neurodegenerative disorders.
Keyphrases
- reactive oxygen species
- induced apoptosis
- hydrogen peroxide
- oxidative stress
- photodynamic therapy
- diabetic rats
- cell cycle arrest
- dna damage
- cell death
- single cell
- high glucose
- cell therapy
- endoplasmic reticulum stress
- stem cells
- nitric oxide
- poor prognosis
- squamous cell carcinoma
- wound healing
- mesenchymal stem cells
- spinal cord injury
- fluorescence imaging
- bone marrow
- ischemia reperfusion injury
- neoadjuvant chemotherapy
- binding protein
- long non coding rna
- cell proliferation
- electron transfer
- heat shock protein
- heat stress