Consecutive non-training days over a weekend for assessing cardiac parasympathetic variation in response to accumulated exercise stress.

Purpose: To examine the association between day-to-day resting cardiac parasympathetic variability over consecutive non-training days (i.e. weekend) and accumulated exercise stress when quantified using indices of cardiovascular strain. Methods: Twelve international calibre female field hockey players training as part of a national team were participants over a four-week mesocycle prior to a 2016 Olympic qualifying tournament. On-field exercise stress was examined using heart rate (HR) dynamics and quantified as; (1) training load and (2) time (min) spent above anaerobic threshold. The square root of the mean squared differences of successive cardiac cycles (R-R intervals) recorded on Saturday and Sunday were individually calculated and log-transformed prior to being averaged (Ln rMSSDweekend). Day-to-day variation in Ln rMSSD over the weekend was expressed using the coefficient of variation (Ln rMSSDCV). Non-linear regression analysis examined the association between accumulated exercise stress and Ln rMSSDCV. Results: A quadratic association between each index of exercise stress and Ln rMSSDCV was identified. After converting the coefficient of determination into a correlation coefficient (90% CL), the respective association between Ln rMSSDCV and training load (AU); r = 0.40 (0.16:0.59) and time above threshold; r = 0.35 (0.06:0.59) were observed. Conclusion: Ln rMSSDCV derived over consecutive non-training days displayed a moderate, yet significant association between accumulated exercise stress when expressed as global or high-intensity indices of cardiovascular strain. Weekend assessments may offer a practical and appropriate juncture between microcycles to assess the magnitude of perturbation in cardiac autonomic homeostasis prior to entering subsequent training periods.