An easy and efficient permeabilization protocol for in vivo enzyme activity assays in cyanobacteria.
Randi Engelberth RasmussenSimon Matthé ErstadErick Miguel Ramos-MartinezLorenzo FimognariAlice Jara De PorcellinisYumiko SakuragiPublished in: Microbial cell factories (2016)
An easy, efficient and scalable permeabilization protocol was established for cyanobacteria. The permeabilized cells can be directly applied for measurement of G6PDH and Rubisco activities without using radioisotopes and the protocol may be readily adapted to studies of other cyanobacterial species and other intracellular enzymes. The permeabilization and enzyme assays can be performed in 96-well plates in a high-throughput manner.