A modified protocol for successful miRNA profiling in human precision-cut lung slices (PCLS).
Monika NiehofStella Marie Reamon-BuettnerOlga DanovTanja HansenKatherina SewaldPublished in: BMC research notes (2021)
The modified method relies on the separation of lysis and precipitation steps, and a clean-up procedure with specific magnetic beads. We obtained successfully quality miRNA amenable for downstream applications such as RTqPCR and whole transcriptome miRNA analysis. Comparison of miRNA profiles in PCLS with published data from human lung, identified all important miRNAs regulated in IPF, COPD, asthma or lung cancer. Therefore, this shows suitability of the method for analyzing miRNA targets and biomarkers in the valuable human PCLS model.
Keyphrases
- endothelial cells
- chronic obstructive pulmonary disease
- randomized controlled trial
- induced pluripotent stem cells
- lung function
- single cell
- gene expression
- transcription factor
- electronic health record
- mass spectrometry
- machine learning
- genome wide
- high resolution
- liquid chromatography
- air pollution
- molecularly imprinted
- simultaneous determination