Conserved Opposite Functions in Plant Resistance to Biotrophic and Necrotrophic Pathogens of the Immune Regulator SRFR1.
Geon Hui SonJiyun MoonRahul Mahadev ShelakeUyen Thi VuongRobert A IngleWalter GassmannJae-Yean KimSang Hee KimPublished in: International journal of molecular sciences (2021)
Plant immunity is mediated in large part by specific interactions between a host resistance protein and a pathogen effector protein, named effector-triggered immunity (ETI). ETI needs to be tightly controlled both positively and negatively to enable normal plant growth because constitutively activated defense responses are detrimental to the host. In previous work, we reported that mutations in SUPPRESSOR OF rps4-RLD1 (SRFR1), identified in a suppressor screen, reactivated EDS1-dependent ETI to Pseudomonas syringae pv. tomato (Pto) DC3000. Besides, mutations in SRFR1 boosted defense responses to the generalist chewing insect Spodoptera exigua and the sugar beet cyst nematode Heterodera schachtii. Here, we show that mutations in SRFR1 enhance susceptibility to the fungal necrotrophs Fusarium oxysporum f. sp. lycopersici (FOL) and Botrytis cinerea in Arabidopsis. To translate knowledge obtained in AtSRFR1 research to crops, we generated SlSRFR1 alleles in tomato using a CRISPR/Cas9 system. Interestingly, slsrfr1 mutants increased expression of SA-pathway defense genes and enhanced resistance to Pto DC3000. In contrast, slsrfr1 mutants elevated susceptibility to FOL. Together, these data suggest that SRFR1 is functionally conserved in both Arabidopsis and tomato and functions antagonistically as a negative regulator to (hemi-) biotrophic pathogens and a positive regulator to necrotrophic pathogens.
Keyphrases
- plant growth
- transcription factor
- dendritic cells
- crispr cas
- gram negative
- cell wall
- genome wide identification
- antimicrobial resistance
- regulatory t cells
- binding protein
- innate immune
- healthcare
- protein protein
- genome editing
- high throughput
- magnetic resonance imaging
- genome wide
- electronic health record
- multidrug resistant
- dna methylation
- small molecule
- staphylococcus aureus
- type iii
- computed tomography
- pseudomonas aeruginosa
- machine learning
- big data
- artificial intelligence
- long non coding rna
- candida albicans
- immune response
- deep learning
- wild type
- contrast enhanced