Inhibition of osteoblast proliferation and migration by exogenous and endogenous formaldehyde.
Xu TengPei WangTianshu YangWei HuangHefeng YuWeishi LiZhongqiang ChenDongwei FanPublished in: Human & experimental toxicology (2020)
Exogenous and endogenous formaldehyde (FA) both play an important role in cell growth and migration; however, their potential role in osteoblasts remains largely unclear. Cell counting kit-8 (CCK-8) and wound-healing assays revealed that FA exposure at naturally occurring concentrations inhibited the proliferation and migration of mouse preosteoblast MC3T3-E1 cells. Moreover, RNA sequencing (RNA-seq) analysis revealed that FoxO1 signaling pathway components displayed distinct expression patterns upon FA exposure, reflected through significant enrichment of cell migration. In particular, FoxO1-, Sirt1-, and FA-induced protein expression, which was closely associated with cell proliferation and migration, was confirmed by western blotting. The results obtained indicated that the FoxO1 pathway is involved in FA-induced inhibition of cell growth and migration.
Keyphrases
- single cell
- rna seq
- signaling pathway
- pi k akt
- high throughput
- induced apoptosis
- cell migration
- transcription factor
- cell cycle arrest
- diabetic rats
- high glucose
- wound healing
- oxidative stress
- poor prognosis
- room temperature
- epithelial mesenchymal transition
- stem cells
- mesenchymal stem cells
- cell proliferation
- endoplasmic reticulum stress
- binding protein
- endothelial cells
- stress induced