Dgcr8 deletion in the primitive heart uncovered novel microRNA regulating the balance of cardiac-vascular gene program.
Xi ChenLin WangRujin HuangHui QiuPeizhe WangDaren WuYonglin ZhuJia MingYangming WangJianbin WangJie NaPublished in: Protein & cell (2018)
Primitive mammalian heart transforms from a single tube to a four-chambered muscular organ during a short developmental window. We found that knocking out global microRNA by deleting Dgcr8 microprocessor in Mesp1 cardiovascular progenitor cells lead to the formation of extremely dilated and enlarged heart due to defective cardiomyocyte (CM) differentiation. Transcriptome analysis revealed unusual upregulation of vascular gene expression in Dgcr8 cKO hearts. Single cell RNA sequencing study further confirmed the increase of angiogenesis genes in single Dgcr8 cKO CM. We also performed global microRNA profiling of E9.5 heart for the first time, and identified that miR-541 was transiently highly expressed in E9.5 hearts. Interestingly, introducing miR-541 back into microRNA-free CMs partially rescued their defects, downregulated angiogenesis genes and significantly upregulated cardiac genes. Moreover, miR-541 can target Ctgf and inhibit endothelial function. Our results suggest that microRNAs are required to suppress abnormal angiogenesis gene program to maintain CM differentiation.
Keyphrases
- wound healing
- single cell
- cell proliferation
- genome wide
- genome wide identification
- long non coding rna
- gene expression
- heart failure
- rna seq
- long noncoding rna
- dna methylation
- atrial fibrillation
- left ventricular
- genome wide analysis
- quality improvement
- poor prognosis
- copy number
- transcription factor
- high throughput
- bioinformatics analysis
- signaling pathway
- endothelial cells
- angiotensin ii
- vascular endothelial growth factor