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Fast and accurate long-read assembly with wtdbg2.

Jue RuanHeng Li
Published in: Nature methods (2019)
Existing long-read assemblers require thousands of central processing unit hours to assemble a human genome and are being outpaced by sequencing technologies in terms of both throughput and cost. We developed a long-read assembler wtdbg2 (https://github.com/ruanjue/wtdbg2) that is 2-17 times as fast as published tools while achieving comparable contiguity and accuracy. It paves the way for population-scale long-read assembly in future.
Keyphrases
  • single molecule
  • endothelial cells
  • gene expression
  • randomized controlled trial
  • high resolution
  • current status
  • genome wide
  • mass spectrometry