Fucoidan Ameliorates Hepatocellular Carcinoma Induced in Rats: Effect on miR143 and Inflammation.
Yousra M El-FarAhmed E KhodirZiad A EmarahMohamed A EbrahimMohammed M H Al-GayyarPublished in: Nutrition and cancer (2020)
Fucoidan is sulfated polysaccharide of brown seaweed. It offers various pharmacological actions like anti-inflammatory, anti-bacterial and anti-tumor activities. Therefore, we aimed to investigate the effect of targeting microRNA-143 and inflammatory pathway by Fucoidan on experimentally induced hepatocellular carcinoma (HCC) in rats. HCC is experimentally induced in Sprague Dawley by thioacetamide. Rats were treated with 100 mg/kg and 200 mg/kg Fucoidan. Hepatic sections were stained with hematoxylin/esosin for investigation of cell integrity. Moreover, hepatic sections were immunohistochemically stained with antibodies for ki67, TNF-α, and IL-1β. Finally, hepatic tissues were investigated for expression of miR-143, NF-κB, TNF-α, and IL-1β. We found that treating HCC with Fucoidan significantly reduced HCC-induced elevation in oxidative stress. Moreover, Fucoidan reduced HCC-induced in expression of miR-143, NF-κB, TNF-α, and IL-1β. Finally, Fucoidan attenuated pseudohepatic lobules, broad fibrous septa and vacuolar to ballooning degeneration associated with reduction of immunostaining of ki67, TNF-α, and IL-1β. Fucoidan elevated the survival of HCC rats and reduced their serum AFP. In addition, Fucoidan treatment revealed reduction in the expression of miR-143 associated with antioxidant and anti-inflammatory activities in HCC rats.
Keyphrases
- oxidative stress
- diabetic rats
- long non coding rna
- high glucose
- cell proliferation
- poor prognosis
- rheumatoid arthritis
- anti inflammatory
- long noncoding rna
- drug induced
- signaling pathway
- stem cells
- endothelial cells
- squamous cell carcinoma
- single cell
- dna damage
- pi k akt
- gene expression
- cancer therapy
- mass spectrometry
- mesenchymal stem cells
- immune response
- neoadjuvant chemotherapy
- drug delivery
- lymph node
- toll like receptor
- rectal cancer
- induced apoptosis
- stress induced
- heat stress