Novel homologous lactate transporter improves L-lactic acid production from glycerol in recombinant strains of Pichia pastoris.
Pollyne Borborema Almeida de LimaKelly Cristina Leite MulderNadiele Tamires Moreira MeloLucas Silva CarvalhoGisele Soares MeninoEduardo MulinariVirgilio H de CastroThaila F Dos ReisGustavo Henrique GoldmanBeatriz Simas MagalhãesNádia Skorupa ParachinPublished in: Microbial cell factories (2016)
We showed that P. pastoris has a great potential as a fermentative organism for producing L-lactic acid using glycerol as the carbon source at limited oxygenation conditions (below 0.05 % DO in the bioreactor). The best strain had both the LDHb and the homologous lactate transporter encoding genes expressed, and reached a titer 1.5 times higher than the strain with the S. cerevisiae transporter. Finally, it was also shown that increased lactic acid production was concomitant to reduction of acetic acid formation by half.