Virus-like Particle (VLP) Mediated Antigen Delivery as a Sensitization Tool of Experimental Allergy Mouse Models.
Juhyung KimJeein OhChon-Sik KangYoun Soo ChoiPublished in: Immune network (2020)
Antigen delivery systems play critical roles in determining the quality and quantity of Ab responses in vivo. Induction of protective antibodies by B cells is essential in the development of vaccines against infectious pathogens, whereas production of IgE antibodies is prerequisite for investigation of allergic responses, or type 1 hypersensitivity reactions. Virus-like particles (VLPs) are efficient platforms for expression of proteins of interest in highly repetitive manners, which grants strong Ab responses to target antigens. Here, we report that delivery of hen egg lysozyme (HEL), a model allergen, through VLP could provoke strong HEL specific IgE Ab responses in mice. Moreover, acute allergic responses were robustly induced in the mice sensitized with VLPs that express HEL, when challenged with recombinant HEL protein. Our data show that antigen delivery in the context of VLPs could function as a platform for sensitization of mice and for subsequent examination of allergic reactions to molecules of interest.
Keyphrases
- high fat diet induced
- drug induced
- allergic rhinitis
- poor prognosis
- mouse model
- atopic dermatitis
- high frequency
- immune response
- adipose tissue
- skeletal muscle
- high throughput
- respiratory failure
- insulin resistance
- acute respiratory distress syndrome
- extracorporeal membrane oxygenation
- hepatitis b virus
- data analysis
- protein protein
- disease virus