Rapid identification of mcr- 1-positive Escherichia coli from patient urine using a novel lipid-based MALDI-TOF-MS assay.
Richard D SmithJerilyn R IzacMichael HaHyojik YangJennifer K JohnsonRobert K ErnstPublished in: Access microbiology (2021)
Mobilized colistin resistance ( mcr ) genes confer resistance to colistin, a last-resort antibiotic for multidrug-resistant Gram-negative infections. In this case report, we describe a novel lipid-based matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) diagnostic used to rapidly identify an mcr-1 -positive Escherichia coli directly from a patient with a urinary tract infection without the need for ex vivo growth.
Keyphrases
- multidrug resistant
- escherichia coli
- gram negative
- klebsiella pneumoniae
- case report
- acinetobacter baumannii
- urinary tract infection
- drug resistant
- mass spectrometry
- biofilm formation
- bioinformatics analysis
- fatty acid
- peripheral blood
- high throughput
- pseudomonas aeruginosa
- staphylococcus aureus
- transcription factor