Mechanically stable solvent-free lipid bilayers in nano- and micro-tapered apertures for reconstitution of cell-free synthesized hERG channels.
Daisuke TadakiDaichi YamauraShun ArakiMiyu YoshidaKohei ArataTakeshi OhoriKen-Ichi IshibashiMiki KatoTeng MaRyusuke MiyataYuzuru TozawaHideaki YamamotoMichio NiwanoAyumi Hirano-IwataPublished in: Scientific reports (2017)
The self-assembled bilayer lipid membrane (BLM) is the basic component of the cell membrane. The reconstitution of ion channel proteins in artificially formed BLMs represents a well-defined system for the functional analysis of ion channels and screening the effects of drugs that act on them. However, because BLMs are unstable, this limits the experimental throughput of BLM reconstitution systems. Here we report on the formation of mechanically stable solvent-free BLMs in microfabricated apertures with defined nano- and micro-tapered edge structures. The role of such nano- and micro-tapered structures on the stability of the BLMs was also investigated. Finally, this BLM system was combined with a cell-free synthesized human ether-a-go-go-related gene channel, a cardiac potassium channel whose relation to arrhythmic side effects following drug treatment is well recognized. Such stable BLMs as these, when combined with a cell-free system, represent a potential platform for screening the effects of drugs that act on various ion-channel genotypes.
Keyphrases
- cell free
- ionic liquid
- circulating tumor
- endothelial cells
- high resolution
- drug induced
- molecular dynamics simulations
- left ventricular
- high throughput
- genome wide
- heart failure
- gene expression
- adverse drug
- combination therapy
- dna methylation
- atomic force microscopy
- mass spectrometry
- climate change
- single cell
- replacement therapy
- pluripotent stem cells
- solar cells
- single molecule
- electronic health record