A compact regulatory RNA element in human Hsp70 mRNA.

Hsp70 performs molecular chaperone functions by assisting in folding newly synthesized or misfolded proteins, thereby counteracting various cell stresses and preventing multiple diseases including neurodegenerative disorders and cancer. It is well established that Hsp70 upregulation during post-heat shock stimulus is mediated by cap-dependent translation. However, the molecular mechanisms of Hsp70 expression during heat shock stimulus remains elusive, even though the 5’ end of Hsp70 mRNA may form a compact structure to positively regulate protein expression in the mode of cap-independent translation. The minimal truncation which can fold to a compact structure was mapped and its secondary structure was characterized by chemical probing. The predicted model revealed a highly compact structure with multiple stems. Including the stem where the canonical start codon is located, several stems were identified to be vital for RNA folding, thereby providing solid structural basis for future investigations on the function of this RNA structure on Hsp70 translation during heat shock.