Curcumin prevents high glucose damage in retinal pigment epithelial cells through ERK1/2-mediated activation of the Nrf2/HO-1 pathway.
Claudio BucoloFilippo DragoRosa MaistoGiovanni Luca RomanoVelia D'AgataGrazia MaugeriSalvatore GiuntaPublished in: Journal of cellular physiology (2019)
To study the effects of curcumin on human retinal pigment epithelial (RPE) cells exposed to high glucose (HG) insult, we performed in vitro studies on RPE cells cultured both in normal and HG conditions to assess the effects of curcumin on the cell viability, nuclear factor erythroid 2-related factor 2 (Nrf2) expression, HO-1 activity, and ERK1/2 expression. RPE cells exposed to HG insult were treated with curcumin. The cell viability, apoptosis, HO-1 activity, ERK, and Nrf2 expression were evaluated. The data indicated that treatment with curcumin caused a significant decrease in terms of apoptosis. Further, curcumin was able to induce HO-1 expression via Nrf2 activation and counteracts the damage elicited by HG. The present study demonstrated that curcumin provides protection against HG-induced damage in RPE cells through the activation of Nrf2/HO-1 signaling that involves the ERK pathway, suggesting that curcumin may have therapeutic value in the treatment of diabetic retinopathy.
Keyphrases
- living cells
- cell cycle arrest
- pi k akt
- oxidative stress
- induced apoptosis
- high glucose
- endothelial cells
- signaling pathway
- poor prognosis
- cell death
- endoplasmic reticulum stress
- diabetic retinopathy
- cell proliferation
- nuclear factor
- replacement therapy
- smoking cessation
- big data
- electronic health record
- aqueous solution