Colocation of Lipids, Drugs, and Metal Biomarkers Using Spatially Resolved Lipidomics with Elemental Mapping.
Holly-May LewisCatia CostaVeronique Anne DartoisFirat KayaMark ChambersJanella de JesusVladimir PalitsinRoger WebbMelanie Jane BaileyPublished in: Analytical chemistry (2022)
Elemental imaging is widely used for imaging cells and tissues but rarely in combination with organic mass spectrometry, which can be used to profile lipids and measure drug concentrations. Here, we demonstrate how elemental imaging and a new method for spatially resolved lipidomics (DAPNe-LC-MS, based on capillary microsampling and liquid chromatography mass spectrometry) can be used in combination to probe the relationship between metals, drugs, and lipids in discrete areas of tissues. This new method for spatial lipidomics, reported here for the first time, has been applied to rabbit lung tissues containing a lesion (caseous granuloma) caused by tuberculosis infection. We demonstrate how elemental imaging with spatially resolved lipidomics can be used to probe the association between ion accumulation and lipid profiles and verify local drug distribution.
Keyphrases
- high resolution
- mass spectrometry
- liquid chromatography
- gene expression
- fatty acid
- induced apoptosis
- mycobacterium tuberculosis
- emergency department
- cell proliferation
- signaling pathway
- hepatitis c virus
- hiv aids
- risk assessment
- drug induced
- tandem mass spectrometry
- ms ms
- human immunodeficiency virus
- photodynamic therapy
- endoplasmic reticulum stress
- drinking water