Anti-Inflammatory Lignans from the Roots of Asarum heterotropoides var. mandshuricum and Their Mechanism of Action.
Thi Thanh LeManh Tuan HaKang-Hyun HanYong-Bum KimJeong Ah KimByung-Sun MinPublished in: Chemistry & biodiversity (2022)
Bioassay-guided fractionation of Asarum heterotropoides var. mandshuricum F. Maekawa (Aristolochiaceae) root extract led to the isolation and characterization of one new ferulic acid glucose ester (1) and nine known lignans (2-10). Their structures were elucidated using extensive spectroscopic methods, including 1D and 2D NMR, and MS spectra. The anti-inflammatory effects of the isolated compounds were investigated via their inhibition against nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated RAW264.7 mouse macrophage cells. Among them, compound 7 ((1R,2S,5R,6R)-5'-O-methylpluviatilol) showed the most effective inhibitory activity against NO production and expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) protein in an exceedingly dose-dependent manner. In addition, further study revealed that the mechanism of anti-inflammatory activity of the most active lignan (7) might be associated with the inhibition of extracellular-signal-regulated kinase (ERK) and nuclear factor kappa B (NF-κB) phosphorylation.
Keyphrases
- nitric oxide synthase
- nuclear factor
- anti inflammatory
- nitric oxide
- toll like receptor
- inflammatory response
- induced apoptosis
- signaling pathway
- high resolution
- hydrogen peroxide
- poor prognosis
- cell cycle arrest
- mass spectrometry
- protein kinase
- lps induced
- multiple sclerosis
- pi k akt
- binding protein
- immune response
- magnetic resonance
- molecular docking
- ms ms
- transcription factor
- protein protein
- tyrosine kinase
- endoplasmic reticulum stress
- density functional theory
- single cell
- amino acid
- long non coding rna
- cell death
- small molecule
- molecular dynamics simulations
- solid state