Antioxidant and Antibacterial Properties of a Functional Sports Beverage Formulation.
Alexandros KolonasPatroklos VareltzisSmaro KiroglouNikolaos GoutzourelasDimitrios StagosVarvara TrachanaChristina TsadilaDimitris MossialosStamatis MourtakosOlga GortziPublished in: International journal of molecular sciences (2023)
Athletes often consume functional beverages in order to improve performance and reduce oxidative stress caused by high-intensity exercise. The present study aimed to evaluate the antioxidant and antibacterial properties of a functional sports beverage formulation. The beverage's antioxidant effects were assessed on human mesenchymal stem cells (MSCs) by determining thiobarbituric acid reactive substances (TBARS; TBARS levels decreased significantly by 52.67% at 2.0 mg/mL), total antioxidant capacity (TAC; TAC levels increased significantly by 80.82% at 2.0 mg/mL) and reduced glutathione (GSH; GSH levels increased significantly by 24.13% at 2.0 mg/mL) levels. Furthermore, the beverage underwent simulated digestion following the INFOGEST protocol to assess its oxidative stability. The analysis of the total phenolic content (TPC) using the Folin-Ciocalteu assay revealed that the beverage contained a TPC of 7.58 ± 0.066 mg GAE/mL, while the phenolics identified by HPLC were catechin (2.149 mg/mL), epicatechin (0.024 mg/mL), protocatechuic acid (0.012 mg/mL), luteolin 7-glucoside (0.001 mg/mL), and kaempferol-3-O-β-rutinoside (0.001 mg/mL). The beverage's TPC was strongly correlated with TAC (R 2 = 896). Moreover, the beverage showcased inhibitory and bacteriostatic effects against Staphylococcus aureus and Pseudomonas aeruginosa . Lastly, the sensory acceptance test demonstrated that the functional sports beverage was well accepted by the assessors.
Keyphrases
- oxidative stress
- high intensity
- mesenchymal stem cells
- staphylococcus aureus
- pseudomonas aeruginosa
- anti inflammatory
- stem cells
- drug delivery
- dna damage
- umbilical cord
- drug resistant
- mass spectrometry
- silver nanoparticles
- drinking water
- acinetobacter baumannii
- high performance liquid chromatography
- induced apoptosis
- methicillin resistant staphylococcus aureus
- diabetic rats
- liquid chromatography