Detection of Volatile Constituents from Food Lures by Tephritid Fruit Flies.
Tibebe Dejene BiasazinHaimanot Teklemariam ChernetSebastian Larsson HerreraMarie BengtssonMiriam Frida KarlssonJoelle Kristin Lemmen-LecheltTeun DekkerPublished in: Insects (2018)
Tephritid fruit flies require protein for sexual and gonotrophic development. Food-based lures are therefore widely used in strategies to detect and control fruit flies in the Tephritidae family. However, these baits are attractive to a broad range of insect species. We therefore sought to identify volatiles detected by the fly antennae, with the goal to compose lures that more specifically target tephritids. Using gas chromatography-coupled electroantennographic detection (GC-EAD) we screened for antennal responses of four important tephritid species to volatile compounds from five commercially available protein-based baits. Antennal active compounds were reconstituted in synthetic blends for each species and used in behavioral assays. These species-based blends were attractive in olfactometer experiments, as was a blend composed of all antennally active compounds from all the four species we observed (tested only in Bactrocera dorsalis, Hendel). Pilot field tests indicate that the blends need to be further evaluated and optimized under field conditions.
Keyphrases
- gas chromatography
- mass spectrometry
- tandem mass spectrometry
- gas chromatography mass spectrometry
- genetic diversity
- randomized controlled trial
- mental health
- clinical trial
- binding protein
- high resolution mass spectrometry
- amino acid
- high throughput
- human health
- real time pcr
- high resolution
- loop mediated isothermal amplification
- study protocol
- label free
- high density