We determined the cellular responses of human colon cancer HT29 and HCT116 cells to the treatments of nobiletin (NBT) and 5-demethylnobiletin (5DN) using Raman microscopy. Evaluation at both single cell and cell population levels revealed found that NBT induced more changes in the peak intensity of nucleic acid than 5DN, whereas 5DN induced more changes in the peak intensity of localized lipid than NBT. This result indicates the different modes of inhibitory action of these two PMFs against colon cancer cells. Between the two colon cancer cells tested, HCT116 cells were more sensitive to both PMFs than HT29 cells. The Raman data were generally in a good agreement with the flow cytometry data. Our results demonstrate that Raman microscopy is able to provide macromolecular information on cellular responses to anticancer treatments.
Keyphrases
- label free
- cell cycle arrest
- induced apoptosis
- single cell
- high resolution
- high throughput
- single molecule
- nucleic acid
- cell death
- flow cytometry
- endothelial cells
- endoplasmic reticulum stress
- high speed
- high glucose
- optical coherence tomography
- diabetic rats
- stem cells
- high intensity
- oxidative stress
- healthcare
- artificial intelligence
- mass spectrometry
- electron microscopy
- health information