Prostate cancer (PCa) represents a substantial global health concern and a prominent contributor to male cancer-related mortality. The aim of this study is to explore the role of B-type endothelin receptor (EDNRB) in PCa and evaluate its therapeutic potential. The investigation employed predictive methodologies encompassing data acquisition from the GEO and TCGA databases, gene screening, enrichment analysis, in vitro experiments involving PCR, Western blotting, wound healing, and Transwell assays, as well as animal experiments. Analysis revealed a significant downregulation of EDNRB expression in PCa cells. Overexpression of EDNRB demonstrated inhibitory effects on tumor cell growth, migration, and invasion, likely mediated through activation of the cGMP-Protein Kinase G pathway. In vivo experiments further confirmed the tumor-suppressive properties of EDNRB overexpression. These findings underscore the prospect of EDNRB as a therapeutic target for PCa, offering novel avenues for PCa treatment strategies.
Keyphrases
- artificial intelligence
- machine learning
- prostate cancer
- protein kinase
- global health
- cell proliferation
- nitric oxide
- signaling pathway
- public health
- transcription factor
- induced apoptosis
- type diabetes
- high throughput
- cell cycle arrest
- binding protein
- gene expression
- radical prostatectomy
- electronic health record
- risk factors
- south africa
- cardiovascular events
- dna methylation
- coronary artery disease
- oxidative stress
- data analysis