Effects of calcium silicate-based cements on odonto/osteogenic differentiation potential in mesenchymal stem cells.
Soner SismanogluPinar ErcalPublished in: Australian endodontic journal : the journal of the Australian Society of Endodontology Inc (2022)
The objective of this study was to evaluate the biological effects and odonto/osteogenic differentiation potential of Biodentine, NeoMTA Plus and TheraCal LC in tooth germ-derived stem cells (TGSCs). TGSCs were exposed to the material extracts. Biocompatibility was tested with MTS cell proliferation assay. Odonto/osteogenic differentiation was assessed with alkaline phosphatase (ALP) activity and mRNA gene expressions (RUNX2, DSPP and DMP-1). Scanning electronic microscopy/energy-dispersive X-ray (SEM/EDX) analysis and pH analysis were also performed for the materials. Data were evaluated using the one-way ANOVA and Tukey's tests. TGSCs remained viable after 7 days of incubation with all tested materials. Biodentine and NeoMTA Plus showed high ALP activity and increased expression of RUNX2, DSPP and DMP-1 compared to that of TheraCal LC. All materials can induce odonto/osteogenic differentiation of MSCs in various levels. Biocompatibility and odonto/osteogenic differentiation potential of Biodentine and NeoMTA Plus are similar and superior to that of TheraCal LC.
Keyphrases
- mesenchymal stem cells
- umbilical cord
- bone marrow
- stem cells
- high resolution
- cell proliferation
- cell therapy
- simultaneous determination
- transcription factor
- solid phase extraction
- poor prognosis
- mass spectrometry
- magnetic resonance imaging
- machine learning
- binding protein
- magnetic resonance
- liquid chromatography
- single molecule
- genome wide
- high speed
- signaling pathway
- copy number
- gene expression
- risk assessment
- deep learning
- cell cycle
- dna methylation
- climate change
- optical coherence tomography
- artificial intelligence
- label free
- long non coding rna
- genome wide identification