Login / Signup

A new staining protocol for detection of murine antibody-secreting plasma cell subsets by flow cytometry.

Katharina PrachtJulia MeinzingerPatrick DaumSebastian R SchulzDorothea ReimerManuela HaukeEdith RothDirk MielenzClaudia BerekJoana Côrte-RealHans-Martin JäckWolfgang Schuh
Published in: European journal of immunology (2017)
We provide a robust four-color fluorescence-based flow cytometry protocol that distinguishes viable dividing plasmablasts from nondividing plasma cells and, based on CD19 surface abundance, identifies two mature plasma cell populations in the spleen and the bone marrow of mice.
Keyphrases