A new staining protocol for detection of murine antibody-secreting plasma cell subsets by flow cytometry.
Katharina PrachtJulia MeinzingerPatrick DaumSebastian R SchulzDorothea ReimerManuela HaukeEdith RothDirk MielenzClaudia BerekJoana Côrte-RealHans-Martin JäckWolfgang SchuhPublished in: European journal of immunology (2017)
We provide a robust four-color fluorescence-based flow cytometry protocol that distinguishes viable dividing plasmablasts from nondividing plasma cells and, based on CD19 surface abundance, identifies two mature plasma cell populations in the spleen and the bone marrow of mice.
Keyphrases
- flow cytometry
- bone marrow
- single cell
- randomized controlled trial
- cell therapy
- induced apoptosis
- mesenchymal stem cells
- genome wide
- cell cycle arrest
- single molecule
- oxidative stress
- cell death
- cell proliferation
- adipose tissue
- microbial community
- dna methylation
- endoplasmic reticulum stress
- metabolic syndrome
- high fat diet induced
- wastewater treatment
- label free
- sensitive detection