A Trp-BODIPY cyclic peptide for fluorescence labelling of apoptotic bodies.
Ramon Subiros-FunosasLorena Mendive-TapiaJesus SotJohn D PoundNicole BarthYaiza VarelaFelix M GoñiMargaret PatersonChristopher D GregoryFernando AlbericioIan DransfieldRodolfo LavillaMarc VendrellPublished in: Chemical communications (Cambridge, England) (2018)
The rational design and synthesis of a Trp-BODIPY cyclic peptide for the fluorescent labelling of apoptotic bodies is described. Affinity assays, confocal microscopy and flow cytometry analysis confirmed the binding of the peptide to negatively-charged phospholipids associated with apoptosis, and its applicability for the detection and characterisation of subcellular structures released by apoptotic cells.