Phenotypic and Genotypic Characterization of Recently Isolated Multidrug-Resistant Acinetobacter baumannii Clinical and Aquatic Strains and Demonstration of Silver Nanoparticle Potency.
Irina Gheorghe-BarbuViorica Maria CorbuCorneliu Ovidiu VrâncianuIoana Cristina MarinașMarcela PopaAndreea Ștefania DumbravăMihai Niță-LazărIonut PeceteAndrei Alexandru MunteanMircea Ioan PopaLiliana MarinescuDenisa FicaiAnton FicaiIlda Czobor BarbuPublished in: Microorganisms (2023)
This study aims to demonstrate the effectiveness of silver nanoparticles (Ag NPs) on multidrug-resistant (MDR) Acinetobacter baumannii (AB) strains isolated from the clinical and aquatic environment. Three types of Ag NPs were investigated for their antimicrobial, antibiofilm, and antivirulence properties on a total number of 132 AB strains isolated in the same temporal sequence from intra-hospital infections (IHIs), wastewater (WW), and surface water (SW) samples between 2019 and 2022 from different Romanian locations and characterized at the phenotypic and genotypic levels. The comparative analysis of the antimicrobial resistance (AR) profiles according to the isolation source and the geographical location demonstrated a decrease in MDR level in AB recovered from WW samples in 2022 from north-eastern/central/southern regions (N-E/C-W/analyzed strains S): 87.5/60/32.5%. The AB strains were lecithinase, caseinase, amylase, and lipase producers, had variable biofilm formation ability, and belonged to six genotypes associated with the presence of different virulence genes ( ompA , csuE , bap , and bfmS ). The Ag NPs synthesized with the solvothermal method exhibited an inhibitory effect on microbial growth, the adherence capacity to the inert substratum, and on the production of soluble virulence factors. We report here the first description of a powerful antibacterial agent against MDR AB strains circulating between hospitals and anthropically polluted water in Romania.
Keyphrases
- multidrug resistant
- acinetobacter baumannii
- escherichia coli
- biofilm formation
- antimicrobial resistance
- drug resistant
- silver nanoparticles
- pseudomonas aeruginosa
- gram negative
- staphylococcus aureus
- klebsiella pneumoniae
- quantum dots
- risk assessment
- randomized controlled trial
- systematic review
- candida albicans
- cystic fibrosis
- gold nanoparticles
- emergency department
- oxide nanoparticles
- highly efficient
- south africa
- adipose tissue
- genome wide
- insulin resistance
- anaerobic digestion