Deepening the role of excitation/inhibition balance in human iPSCs-derived neuronal networks coupled to MEAs during long-term development.

Healthy neuronal activity is controlled by a well-defined E/I balance whose alteration could lead to the onset of neurodevelopmental disorders like schizophrenia or epilepsy. Most of the commonly used in vitro models are animal-derived or too simplified and thus far from the in vivo human condition. In this work, by performing a long-term study of h-iPSCs-derived neuronal networks obtained from healthy human subjects, we demonstrated the feasibility of a robust in vitro model which can be further exploited for investigating pathological conditions where the E/I balance is impaired.&#xD.