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Pseudohyphal growth in Saccharomyces cerevisiae involves protein kinase-regulated lipid flippases.

Merethe Mørch FrøsigSara Rute CostaJohannes LiescheJeppe Thulin ØsterbergSusanne HanischSebastian J NintemannHelle SørensenMichael Broberg PalmgrenThomas Günther PomorskiRosa Laura López-Marqués
Published in: Journal of cell science (2020)
Lipid flippases of the P4 ATPase family establish phospholipid asymmetry in eukaryotic cell membranes and are involved in many essential cellular processes. The yeast Saccharomyces cerevisiae contains five P4 ATPases, among which Dnf3p is poorly characterized. Here, we demonstrate that Dnf3p is a flippase that catalyzes translocation of major glycerophospholipids, including phosphatidylserine, towards the cytosolic membrane leaflet. Deletion of the genes encoding Dnf3p and the distantly related P4 ATPases Dnf1p and Dnf2p results in yeast mutants with aberrant formation of pseudohyphae, suggesting that the Dnf1p-Dnf3p proteins have partly redundant functions in the control of this specialized form of polarized growth. Furthermore, as previously demonstrated for Dnf1 and Dnf2p, the phospholipid flipping activity of Dnf3p is positively regulated by flippase kinase 1 (Fpk1p) and Fpk2p. Phylogenetic analyses demonstrate that Dnf3p belongs to a subfamily of P4 ATPases specific for fungi and are likely to represent a hallmark of fungal evolution.
Keyphrases
  • saccharomyces cerevisiae
  • protein kinase
  • fatty acid
  • mitral valve
  • palliative care
  • cell therapy
  • tyrosine kinase
  • bone marrow
  • atrial fibrillation
  • genome wide analysis